Plasmid

Part:BBa_K4358013:Design

Designed by: HUNG. CHIEN-CHUN   Group: iGEM22_NCHU_Taichung   (2022-10-05)


Multi-pqqA pET-28a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 324
    Illegal XbaI site found at 1681
    Illegal XbaI site found at 1705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 152
    Illegal NheI site found at 619
    Illegal NheI site found at 3473
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 209
    Illegal XhoI site found at 1397
    Illegal XhoI site found at 1997
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 324
    Illegal XbaI site found at 1681
    Illegal XbaI site found at 1705
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 324
    Illegal XbaI site found at 1681
    Illegal XbaI site found at 1705
    Illegal NgoMIV site found at 3655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2609
    Illegal SapI site found at 1807


Design Notes

a


Source

a

References